Hyperglycaemia Attenuated C2C12 Myoblast Proliferation and Induced Skeletal Muscle Atrophy via Modulating Myogenic Regulatory Factors Genes Expression in Diabetic Rats

Diabetes mellitus is characterised by high blood glucose level termed hyperglycaemia (HG). It has been reported to affect skeletal muscle inducing atrophy and protein degradation leading impairment of function. This study aimed investigate the effects HG on expression myogenic regulatory factor genes in progenitor cells muscle. The number C2C12 myoblasts cultured condition was significantly decreased compared control dose time dependent manner. In addition, Ki-67 positive nuclei after treatment under condition. Real PCR showed significant suppression MyoD myogenin, while Myf5 gene enhanced, control. Furthermore, histological examinations fibres tibialis anterior (TA) from diabetic rats. frequency distribution fibre cross-sectional area (MCA) rats shifted leftward that normal contrast, myogenin TA were increased provides novel knowledge changing hyperglycaemic condition, both vitro vivo, atrophy.